|
KMID : 0613820030130020163
|
|
Journal of Life Science
2003 Volume.13 No. 2 p.163 ~ p.167
|
|
|
Molecular probe for identification of cysts of resting cyst of PSP-producer Alexandrium tamarense (Dinophyceae)
|
|
Cho Eun-Seob
|
|
|
Abstract
|
|
|
|
Identification of species within the toxin-producing genus Alexandrium is vital for biotoxin monitoring and mitigation decisions regarding shellfish industry. In particular, the discrimination of resting cysts of only A. tamarense from that of Alexandrium spp. is considerable important to fundamentally monitor and predict this species before vegetative cells occur in the nature. Fluorescent cTAM-F1 DNA probe was responsible to not only binding the activity of the vegetative cells in A. tamarense, but also to the resting cysts, which was treated with methanol after fixation and stained by primuline on the surface. The location of fluorescence in cultured vegetative cells and resting cysts was almost at the bottom of the nucleus. The optimal incubation temperature and time using in situ hybridization were 50～54℃ and 40～60 min, respectively, to penetrate the DNA probe into cell.
|
|
|
KEYWORD
|
|
|
Alexandrium tamarense, Alexandrium catenella, biotoxin monitoring system, Chinhae Bay, DNA probe, red tide, resting cysts
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|